Learn when it's safe to transplant seedlings. The first two leaves visible are usually "cotyledons," or seed leaves. Once the seedling grows several "true leaves," with a more typical rose leaf appearance, it is more likely to survive transplanting. It is also easier on plants if they are transplanted to a larger pot for a year or two, and then transplanted outside.
As soon as the rose seeds have been removed from the rose hips, rinse the seeds in a solution of purified water (any bottled water will do) with 5% bleach. This would be about two teaspoons of bleach per cup of water. Rinse the rose seeds with plain bottled water in a strainer, then soak the seeds in straight 3% peroxide for 24 hours. CAUTION: Do not mix bleach with peroxide because there could be a chemical reaction.
When you grow roses from seed, you are essentially developing new varieties of roses. If the rose fruit -- called a hip -- was produced by bee pollination, you don't know what varieties were crossed to get the seeds. Rose breeders hybridize chosen varieties and select for particular traits in resultant seedlings. However, each seedling will be a surprise. Rose seeds have to be collected in the fall, cleaned, and cold-treated before they are planted. Even though the cleaned seeds may look dry, they are not dry inside. If rose seeds are allowed to sit around in the open air and dry up completely, they will not grow.
Soaking the seeds is a crucial step if your seeds will germinate properly and stay clear of any diseases. You MUST not mix the bleach with the hydrogen peroxide as this results in a chemical reaction. 3% peroxide for 24 hours is just fine. This is also a good time to perform the water float test. Remove all seeds that float as they might not be viable.
Monitor the rose cuttings to ensure they’re hydrated and taking root. Keep an eye on the cuttings to make sure they’re never dried out, as well as to make sure the cuttings are taking root. You can test to see if the roots are growing by gently tugging on the cuttings. You should be able to feel a slight resistance after a week or 2, meaning the roots are growing well.